March 12, 1985Biochemistry

Rate-limiting step in the actomyosin adenosine triphosphatase cycle: experiments with myosin subfragment 1 crosslinked to actin

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Population

Myosin subfragment 1 (S-1) crosslinked to actin

Comparison

Mixing with ATP vs S-1 alone

Design

Preclinical

Authors

LSLeonard A. SteinRutgers, The State University of New Jersey LGLois E. GreeneNational Institutes of Health PCP Boon ChockNational Institutes of Health

Discussion

Member takes

Implication

Biochemical support for acto-S-1-ADP-Pi complexes in the ATPase cycle; leaves open relevance at physiological ionic strength.

Structured PICO

Population

Myosin subfragment 1 (S-1) crosslinked to actin

Intervention

Mixing with ATP

Comparator

S-1 alone

Outcome

Amount of acto-S-1-ADP-Pi formed (measured by intrinsic fluorescence enhancement and direct measurement of Pi)surrogate

The study provides biochemical evidence regarding the formation of acto-S-1-ADP-Pi complexes, contributing to the understanding of the rate-limiting step in the actomyosin ATPase cycle.

Cite This Study

Stein et al. (1985) studied this question.

synapsesocial.com/papers/6a7f3cc8fdae2f46226a7bcd https://doi.org/10.1021/bi00327a013

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Bookmark

Rate-limiting step in the actomyosin adenosine triphosphatase cycle: experiments with myosin subfragment 1 crosslinked to actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Rate-limiting step in the actomyosin adenosine triphosphatase cycle: experiments with myosin subfragment 1 crosslinked to actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider