Characterizations of Adenovirus Type 41 Isolates from Children with Acute Gastroenteritis in Japan, Vietnam, and Korea
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Key Points
To genetically and antigenically characterize adenovirus type 41 (Ad41) isolates collected from pediatric patients with acute gastroenteritis across three Asian nations.
Analyzed 70 Ad41 strains isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea.
Conducted DNA restriction enzyme (RE) profiling, sequencing of the hexon hypervariable regions (HVRs), and phylogenetic analysis.
Evaluated antigenic reactivity using an enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody (MAb) 1F.
Identified eight distinct genome types, including four novel variants (D25, D26, D27, and D28), which clustered into two lineages: GTC1 (D1, D25–D28) and GTC2 (D4, D12, D22).
Observed 97% to 100% intra-cluster amino acid homology within each genome-type cluster versus 92% to 94% inter-cluster homology across the hexon HVRs.
Demonstrated antigenic differentiation between clusters, as monoclonal antibody 1F reacted exclusively with GTC1 isolates and showed no reactivity with GTC2 strains.