What question did this study set out to answer?

To investigate and compare the specific pathways and time courses of eicosanoid and prostaglandin generation in allergen-activated mouse basophils and mast cells.

March 15, 2017Biochemistry and Biophysics ReportsOpen Access

Differential usage of COX-1 and COX-2 in prostaglandin production by mast cells and basophils

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Authors

TBTomoyuki BandoTokyo Medical and Dental University SFSetsuko FujitaTohoku University NNNaoko NaganoNational Center For Child Health and Development

Discussion

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Implication

Comparative laboratory analysis demonstrates divergent COX-1 and COX-2 pathways for prostaglandin production in mouse basophils versus mast cells, indicating distinct regulatory roles during allergy.

Key Points

To investigate and compare the specific pathways and time courses of eicosanoid and prostaglandin generation in allergen-activated mouse basophils and mast cells.
Stimulated mouse basophils and mast cells with IgE plus allergens.
Measured 5-LOX metabolites (LTB4, 5-HETE) and COX metabolites (PGD2, PGE2, 11-HETE) at 0.5 and 6 hours post-stimulation.
Quantified baseline and post-activation expression of COX-1 and COX-2, and tested mediator production under selective COX-1 and COX-2 inhibition.
Mast cells displayed 8- to 9-fold higher resting levels of COX-1 than basophils and produced COX metabolites at both 0.5 and 6 hours post-stimulation, with early-phase production blocked by COX-1 but not COX-2 inhibitors.
Basophils expressed 4- to 5-fold higher levels of COX-2 than mast cells upon activation and produced COX metabolites exclusively at 6 hours post-stimulation, which was completely ablated by COX-2 inhibition.
Both cell types produced 5-LOX metabolites within 0.5 hours of stimulation, though basophils generated substantially lower amounts than mast cells.

Cite This Study

Bando et al. (2017) studied this question.

synapsesocial.com/papers/6a80d79a8412ed78203be3d0 https://doi.org/10.1016/j.bbrep.2017.03.004

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Implication

Comparative laboratory analysis demonstrates divergent COX-1 and COX-2 pathways for prostaglandin production in mouse basophils versus mast cells, indicating distinct regulatory roles during allergy.

Differential usage of COX-1 and COX-2 in prostaglandin production by mast cells and basophils

Discussion

Member takes

Implication

Key Points

Cite This Study

Differential usage of COX-1 and COX-2 in prostaglandin production by mast cells and basophils

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider