What question did this study set out to answer?

To identify and characterize the ionic conductance mechanisms activated by cell swelling during regulatory volume decrease in cultured chick heart cells.

December 1, 1993The Journal of PhysiologyOpen Access

A chloride current associated with swelling of cultured chick heart cells.

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Population

Cultured chick heart cells

Comparison

Hyposmotic stress and application of Cl- channel… vs Isosmotic bath solution

Design

Preclinical

Authors

JZJian ZhangKanazawa University RRRandall L. RasmussonJacobs (United States)SHSarah HallUniversity of Utah

Discussion

Member takes

Implication

Does not support clinical changes; leaves open Cl- channel role in mammalian cardiac volume regulation.

Key Points

To identify and characterize the ionic conductance mechanisms activated by cell swelling during regulatory volume decrease in cultured chick heart cells.
Exposed cultured embryonic chick heart aggregates and single cells to hyposmotic stress induced by NaCl reduction.
Measured membrane potential and whole-cell currents using conventional microelectrode and patch-clamp electrophysiology with potassium currents blocked.
Determined reversal potentials, relative anion permeabilities (chloride, methanesulphonate, and aspartate), and sensitivity to the chloride channel blocker diphenylamine-2-carboxylate (200 µM).
Hyposmotic exposure triggered an initial increase in spontaneous electrical and contractile activity followed by sustained depolarization and quiescence, alongside a fully reversible whole-cell conductance increase.
The reversal potential of the swelling-activated current shifted with chloride concentrations at 51 mV per decade change in [Cl-]i/[Cl-]o (regression slope 0.82 relative to ECl), displaying an anion permeability sequence of Cl- > MSA- >> Asp- (1:0.36:0.02).
Diphenylamine-2-carboxylate (200 µM) substantially inhibited the swelling-induced current across membrane potentials without altering its reversal potential.

Structured PICO

Population

Cultured chick heart cells

Intervention

Hyposmotic stress (NaCl reduction) and application of Cl- channel blocker diphenylamine-2-carboxylate (DPC, 200 microM)

Comparator

Isosmotic bath solution

Outcome

Activation of whole-cell conductance and swelling-activated chloride currentsurrogate

Hyposmotic swelling of cultured chick heart cells activates a channel-mediated chloride conductance likely involved in volume regulation.

Cite This Study

Zhang et al. (1993) studied this question.

synapsesocial.com/papers/6a80d900fd865eccc665dcbd https://doi.org/10.1113/jphysiol.1993.sp019974

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A chloride current associated with swelling of cultured chick heart cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

A chloride current associated with swelling of cultured chick heart cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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