February 1, 2004Journal of Biological ChemistryOpen Access

Molecular Determinants of Na+/Ca2+ Exchange (NCX1) Inhibition by SEA0400

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Population

NCX-transfected fibroblasts and porcine renal tubular cells (hypoxic injury model)

Comparison

SEA0400 (Na+/Ca2+ exchanger inhibitor) vs Untreated cells or cells expressing…

Design

Preclinical

Authors

TITakahiro IwamotoOkayama University SKSatomi KitaJikei University School of Medicine AUAkira UeharaKanazawa University

Discussion

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Implication

SEA0400 may protect renal cells from hypoxic injury via NCX1; leaves open therapeutic translation pending in vivo validation.

Structured PICO

Population

NCX-transfected fibroblasts and porcine renal tubular cells (hypoxic injury model)

Intervention

SEA0400 (Na+/Ca2+ exchanger inhibitor)

Comparator

Untreated cells or cells expressing SEA0400-insensitive mutants

Outcome

Inhibitory effects on Na+(i)-dependent 45Ca2+ uptake and whole-cell Na+/Ca2+ exchange currents, and protection against hypoxia/reoxygenation-induced cell damagesurrogate

Phe-213, Gly-833, and residues that eliminate Na+-dependent inactivation are critical determinants for NCX1 inhibition by SEA0400, which exerts protective effects in a hypoxic injury model.

Cite This Study

Iwamoto et al. (2004) studied this question.

synapsesocial.com/papers/6a813a2e17c102c671648d10 https://doi.org/10.1074/jbc.m310491200

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Molecular Determinants of Na+/Ca2+ Exchange (NCX1) Inhibition by SEA0400

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Molecular Determinants of Na+/Ca2+ Exchange (NCX1) Inhibition by SEA0400

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider