Inducing Metabolic Dysfunction in Breast Cancer Cells Through Targeted Inhibition of α ‐ and β ‐Glucosidase and Amylase: Novel Carbohydrate (C ₈ N) Isomers Designed via Intramolecular aza ‐Michael‐Type Reaction

Natural products such as glucosidase inhibitors and aminoglycoside antibiotics are target agents that play an important role in the development of new drugs. A novel class of glucosidase inhibitors based on aminocyclitols was synthesized using a new method. This method involves arranging the α‐ , β ‐unsaturated structure by attacking the ammonia compound on the acetate group in the γ ‐position forming, to form the amide in the β ‐position. The method has enabled the synthesis of new types of C 8 N, such as C 7 N (C 8 N generally exhibits structural and characteristic biological properties similar to C 7 N) compounds. After purification and structure elucidation of the resulting substances, α ‐, β ‐glucosidase, and amylase tests were performed against acarbose; additionally, an enzyme kinetic study of well‐active compounds was conducted. Subsequently, the anticancer activity of substances was analyzed using a viability assay and AO/PI staining. Our results suggest that different derivatives of new carbosugar isomers exert potential enzymatic and anticancer activity against breast cancer cells.