January 1, 2008Platelets
Reproducibility and standardized reporting of the vasodilator-stimulated phosphoprotein phosphorylation assay
Why the study?
Does using reduced reagent volumes and different fluorescence intensity calculations affect the reproducibility and results of the VASP assay for clopidogrel responsiveness?
Population
280 patients on clopidogrel therapy and 35 healthy volunteers without clopidogrel therapy
Comparison
VASP assay using half or quarter of recommended… vs VASP assay using full recommended reagent…
Design
Cross-sectional
Authors
Discussion
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Member takes
Implication
May support reagent-sparing VASP use for clopidogrel testing; leaves open PRI standardization and prospective validation.
Structured PICO
Does using reduced reagent volumes and different fluorescence intensity calculations affect the reproducibility and results of the VASP assay for clopidogrel responsiveness?
P
Population
280 patients on clopidogrel therapy and 35 healthy volunteers without clopidogrel therapy
I
Intervention
VASP assay using half or quarter of recommended reagent volume; PRI calculation using geometric mean or median fluorescence intensity
C
Comparator
VASP assay using full recommended reagent volume; PRI calculation using mean fluorescence intensity
O
Outcome
Reproducibility of VASP assay with reduced reagent volumes and differences in Platelet Reactivity Index (PRI) based on fluorescence intensity calculation methodsurrogate
Using half the recommended reagent volume for the VASP assay maintains excellent reproducibility and offers a cost-saving strategy, while standardizing PRI calculation methods is necessary to avoid variability.
Cite This Study
Siller‐Matula et al. (2008) studied this question.