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January 24, 2009Clinical ChemistryOpen Access

Comparison of Immunoassays for the Selective Measurement of Human High–Molecular Weight Adiponectin

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Why the study?

Do commercial ELISAs accurately measure HMW adiponectin compared to Western blotting in healthy individuals and patients with CAD or type 2 diabetes?

Population

204 individuals including 83 patients with coronary artery disease, 81 patients with type 2 diabetes, and 40…

Comparison

Measurement of high-molecular-weight adiponectin… vs Quantitative Western blotting

Design

Cross-sectional

Authors

DLDan LiuTSTibor SchusterMBMarcus Baumann

Discussion

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Overview

ELISAs agree closely with Western blotting; supports use in large studies but leaves open broader validation before clinical adoption.

Structured PICO

Do commercial ELISAs accurately measure HMW adiponectin compared to Western blotting in healthy individuals and patients with CAD or type 2 diabetes?

P
Population
204 individuals including 83 patients with coronary artery disease (CAD), 81 patients with type 2 diabetes, and 40 healthy controls
I
Intervention
Measurement of high-molecular-weight (HMW) adiponectin using 2 commercial ELISAs (ALPCO Diagnostics and Millipore)
C
Comparator
Quantitative Western blotting
O
Outcome
Correlations, range of agreement, and imprecision of HMW adiponectin concentrationssurrogate

Commercial ELISAs for HMW adiponectin demonstrate high agreement with Western blotting, supporting their use in large clinical and epidemiological studies.

Cite This Study

Liu et al. (2009) studied this question.

synapsesocial.com/papers/6a81ad8f2ac899ae0206d5aehttps://doi.org/10.1373/clinchem.2008.112425
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