Key result
Sodium heparin and 45 µg/mL r-hirudin provided the longest storage time for impedance aggregometry, maintaining stability up to 12 hours for ADP-induced and 24 hours for AA-induced tests.
Why the study?
Does the choice of anticoagulant and storage temperature affect the stability of whole blood aggregometry platelet function testing?
Does the choice of anticoagulant and storage temperature affect the stability of whole blood aggregometry platelet function testing?
Sodium heparin and 45 µg/mL r-hirudin are the optimal anticoagulants for maximizing the storage time of blood samples for impedance aggregometry.
May guide anticoagulant selection for aggregometry storage in research; leaves open human validation before clinical use.
Major hindrances of impedance aggregometry are caused by limited storage time and the requirement of ex vivo anticoagulation. Data on the influence of different anticoagulants and storage conditions are rare and incomplete. This study has systematically examined the influence of six different anticoagulants (sodium and lithium heparin, 20 µg/mL and 45 µg/mL r-hirudin, benzylsulfonyl-D-Arg-Pro-4-amidinobenzylamide (BAPA), and citrate) on the results of Adenosine 5'-diphosphate (ADP) and arachidonic acid (AA) induced measurements using multiple-electrode impedance aggregometer (MEA). Measurements were carried out in a time frame of 0 min up to 48 h after blood withdrawal. In addition, the influence of storage temperatures of 4°C and 37°C was evaluated. Results of ADP-induced tests significantly varied within the first 30 min in all tested anticoagulants, in citrated blood even within the first 60 min. They remained stable up to 2 h in 20 µg/mL r-hirudin and BAPA, 4 h in citrate, 8 h in 45 µg/mL r-hirudin, and lithium heparin and up to a maximum of 12 h in sodium heparin anticoagulated blood. The analysis of AA-induced tests revealed no significantly different results up to 6 h when BAPA was used, 8 h in lithium heparin, 20 µg/mL r-hirudin and citrate, 12 h in 45 µg/mL r-hirudin, and even 24 h in sodium heparin-anticoagulated samples. A storage temperature of either 4°C or 37°C in contrast to room temperature had a negative influence on the stability of results. In conclusion, sodium heparin and 45 µg/mL r-hirudin as anticoagulants guarantee the longest storage time for impedance aggregometry.
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Kaiser et al. (2011) studied this question. Different anticoagulants (sodium/lithium heparin, r-hirudin, BAPA, citrate) and storage temperatures vs. Each other and room temperature was evaluated on Stability of ADP- and AA-induced platelet aggregation measurements. Sodium heparin and 45 µg/mL r-hirudin provided the longest storage time for impedance aggregometry, maintaining stability up to 12 hours for ADP-induced and 24 hours for AA-induced tests.
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