Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia

Figures

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  Fig. 1. ITLN1 expression is decreased in the left ventricle and circulating plasma levels of ITLN1 are increased following acute ischemia in the human heart. Stripcharts display the change in ITLN1 expression in left ventricular tissue samples (a) and in circulating ITLN1 protein levels following acute ischemia in the heart (b). FPKM: fragments per kilobase per million mapped reads

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  Fig. 2. Venn diagram of differentially regulated genes between subcutaneous adipose tissue (SAT) vs. visceral adipose tissue (VAT) and epicardial adipose tissue (EAT) vs. SAT. In the SAT vs. VAT comparison, 845 genes were differentially regulated (FC > 2.0, p < 0.05). In the EAT vs. SAT comparison 188 genes were differentially regulated. 46% of these were commonly differentially regulated in VAT in comparison to SAT (middle overlapping segment). The numbers in each circle indicate the number of genes whose expression was altered; numbers in bold italic are upregulated, those in red font are contraregulated, and those in underlined plain font are downregulated in the respective comparisons. The specific genes are listed in Table 3

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  Fig. 3. ITLN1 is specifically expressed in the stromal vascular fraction of visceral fat in humans. a Microarrays were queried for expression of ITLN1 in visceral adipose tissue (VAT) vs. subcutaneous adipose tissue (SAT) in paired samples (n = 8) (*** p < 0.001). b ITLN1 expression from microarrays of the adipocyte and stromal vascular fraction (SVF) of VAT in paired samples (n = 6) (** p < 0.01). c Expression of ITLN1 from microarrays of murine SAT and VAT

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  Fig. 4. Immunohistochemistry of ITLN1 in epicardial adipose tissue (EAT) shows enrichment within the mesothelial cell layer. Subcutaneous adipose tissue (SAT) stained for ITLN1 (a). EAT stained for ITLN1 (b) with magnification of the mesothelial cell layer of the visceral pericardium (c). Secondary antibody alone was used as a control (d). ITLN1 red, DAPI blue

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  Fig. 5. ITLN1 expression in primary mesothelial cells is dramatically reduced following an epithelial to mesenchymal transition. a qRT-PCR for ITLN1 in mesothelial cells ± TGFβ1 and in a human adipocyte cell line (SGBS) before (D0) and after differentiation (D14) (n = 6 per group). b qRT-PCR for the adipocyte specific gene PLIN1 in mesothelial cells ± TGFβ1 and in a human adipocyte cell line (SGBS) before (D0) and after differentiation (D14)(n = 6 per group). c Expression of the epithelial cell marker CDH-1 and the mesenchymal cell markers VMAC, SNAI1, and SNAI2 before and after treatment of mesothelial cells with TGFβ1 (n = 6 per group)