What question did this study set out to answer?

To determine whether functional and receptor diversity across vascular endothelial cells from different organs directs the tissue-specific tropism and pathology of coxsackievirus group B type 3.

September 1, 1990Journal of VirologyOpen Access

Functional diversity in vascular endothelial cells: role in coxsackievirus tropism

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Population

Vascular endothelial cells (VEC) of the liver, lungs, and heart; BALB/c mice

Comparison

Infection with coxsackievirus group B type 3… vs Heart-derived versus liver-derived virus isolates

Design

Preclinical

Follow-up

7 days

Authors

SHSally A. HuberUniversity of Vermont CHCarl E. HaischEast Carolina University PLPatricia A. LodgeIsto Biologics (United States)

Discussion

Member takes

Implication

Supports organ-specific endothelial targeting in CVB3 pathogenesis; leaves open translation to human myocarditis and antiviral strategies.

Key Points

To determine whether functional and receptor diversity across vascular endothelial cells from different organs directs the tissue-specific tropism and pathology of coxsackievirus group B type 3.
Assayed six plaque-purified CVB3 isolates from infected DBA/2 mouse heart and liver for in vitro infectivity, replication, and receptor binding in heart, liver, and lung vascular endothelial cells (VEC).
Inoculated BALB/c mice intraperitoneally with 10(4) PFU of heart- or liver-derived viral isolates to evaluate tissue injury and viral titers in hearts, livers, and lungs after 7 days.
Heart-derived virus isolates showed selective binding, higher infectivity, and greater replication in heart VEC in vitro and caused severe myocarditis in vivo.
Liver-derived isolates preferentially infected liver VEC and reinfected liver tissue in vivo but were completely unable to cause myocarditis despite presence in heart tissue.

Structured PICO

Population

Vascular endothelial cells (VEC) of the liver, lungs, and heart; BALB/c mice

Intervention

Infection with coxsackievirus group B type 3 isolates derived from heart or liver tissue

Comparator

Heart-derived versus liver-derived virus isolates

Outcome

Percentage of VEC infected, mean progeny PFU produced per infected VEC in vitro, and tissue injury (myocarditis) and virus concentrations in vivosurrogate

Selective tropism of coxsackievirus isolates for particular organs in vivo corresponds to their ability to infect vascular endothelial cells of those organs in vitro.

Cite This Study

Huber et al. (1990) studied this question.

synapsesocial.com/papers/6a81fd43b35cb7638cee3fa1 https://doi.org/10.1128/jvi.64.9.4516-4522.1990

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Implication

Supports organ-specific endothelial targeting in CVB3 pathogenesis; leaves open translation to human myocarditis and antiviral strategies.

Functional diversity in vascular endothelial cells: role in coxsackievirus tropism

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Functional diversity in vascular endothelial cells: role in coxsackievirus tropism

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider