August 24, 2006Molecular Biology of the CellOpen Access

Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMetTernary Complex Availability

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Population

In vitro translation systems and mouse embryonic fibroblasts, including wild-type and eIF2alpha S51A/S51A…

Comparison

Translation initiation inhibitors NSC119889 and… vs Vehicle (DMSO) control

Design

Preclinical

Authors

FRFrançis RobertMcGill University Health Centre LKLee D. KappUniversity of Pennsylvania SKShakila Nargis KhanUniversity of Dhaka

Discussion

Member takes

Implication

NSC119889 and NSC119893 may spare HCV translation in vivo; leaves open IRES-selective antivirals for further study.

Structured PICO

Population

In vitro translation systems (Krebs-2 extracts) and mouse embryonic fibroblasts (MEFs), including wild-type and eIF2alpha S51A/S51A knockin cells

Intervention

Translation initiation inhibitors NSC119889 and NSC119893

Comparator

Vehicle (DMSO) control

Outcome

Effect on ternary complex formation, 43S preinitiation complex assembly, and HCV IRES-driven versus cap-dependent translationsurrogate

HCV evades the antiviral interferon response by maintaining translation initiation despite reduced availability of the eIF2·GTP·Met-tRNAiMet ternary complex.

Cite This Study

Robert et al. (2006) studied this question.

synapsesocial.com/papers/6a8212610f52950edd99d001 https://doi.org/10.1091/mbc.e06-06-0478

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Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMetTernary Complex Availability

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMetTernary Complex Availability

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider