Key result
Mutant forms of the hepatitis C virus internal ribosome entry site RNA form trapped 48S preinitiation complexes incapable of efficient assembly into 80S ribosomes, showing IRES-eIF3 interaction is required for stable eIF3 and eIF2 association.
Population
Human cell extract (in vitro translation system)
Comparison
Wild-type and mutant hepatitis C virus internal… vs Comparison between wild-type and various mutant…
Design
Preclinical
Authors
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May inform HCV antiviral design targeting IRES-eIF3; leaves open clinical translation from preclinical models.
The HCV IRES RNA coordinates interactions of eIF3 and eIF2 on the ribosome to position the initiator tRNA, providing mechanistic insights into viral translation initiation.
Ji et al. (2004) studied Hepatitis C virus translation initiation. Mutant HCV IRES RNAs vs. Wild-type HCV IRES RNA was evaluated on 80S ribosome assembly and 48S complex formation. Mutant forms of the hepatitis C virus internal ribosome entry site RNA form trapped 48S preinitiation complexes incapable of efficient assembly into 80S ribosomes, showing IRES-eIF3 interaction is required for stable eIF3 and eIF2 association.
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