A novel interspecies recombinant enterovirus (Enterovirus A120) isolated from a case of acute flaccid paralysis in China

Figures

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  Figure 1.. Phylogenetic tree based on the VP1, P1, P2, and P3 sequences of EV-A. Maximum likelihood trees were constructed using the GTR + G model and were implemented in MEGA7.0 with 1000 bootstrap replicates. The circle and square represent the Q0082 strain and the EV-A120 prototype, respectively. Scale bars indicate the genetic distance. All panels have the same proportions. (a) VP1 coding sequence; (b) P1 coding sequence; (c) P2 coding sequence; (d) P3 coding sequence.

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  Figure 2.. Phylogenetic tree based on the full-length genomic sequences of EV-A. Nucleotide sequences of the Q0082 strain (represented by circles) and 23 other EV-A prototype strains were compared using MUSCLE software. A maximum likelihood phylogenetic tree was constructed using MEGA7.0. The branches marked in red represent the prototype strains clustered with the Tibet EV-A120 strain.

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  Figure 3.. Recombination analyses of the Tibet EV-A120 strain with other EV-A strains (a) Recombination events predicted for the Q0082 strains. The genome of the Tibet EV-A120 is shown as a black block. Genetic components identified by RDP4 that were involved in recombination events are shown as light grey blocks. Likely breakpoint positions are shown above the genome. (b) Similarity plots and boot scanning analyses of EV-A120, CV-A2, CV-A4, and CV-A6 strains with potential parents. The Q0082 Strain (EV-A120/Q0082-XZ-CHN-2000) was used as a query sequence; (c) Phylogenetic tree of sequences based on the P1 coding region; (d) Phylogenetic tree of sequences based on the P2 coding region; (e) Phylogenetic tree of sequences based on the P3 coding region. All branches of the trees are coloured according to the results of boot scanning analysis. The sequences analyzed in the recombination and phylogenetic trees were identical.

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  Figure 4.. Temperature sensitivity test curves for the EV-A120 strain. Blue and red lines represent the growth trends of the viruses on RD cells at 36°C and 39.5°C, respectively. The Xinjiang EV-B85 strain (HTYT-ARL-AFP02F/XJ/CHN/2011, showing no temperature sensitivity) and the EV-B106 strain (HTYPS-QDH11F/XJ/CHN/2011, showing temperature sensitivity) were used as experimental controls. (a) Strain EV-A120/Q0082-XZ-CHN-2000; (b) Strain HTYT-ARL-AFP02F/XJ/CHN/2011; (c) Strain HTYPS-QDH11F/XJ/CHN/2011.