What question did this study set out to answer?

To identify and characterize novel endogenous protein inhibitors that regulate human cyclin-dependent kinase (Cdk) complexes during the cell cycle.

June 7, 1994Proceedings of the National Academy of SciencesOpen Access

A cell cycle-regulated inhibitor of cyclin-dependent kinases.

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Authors

LHLudger HengstInnsbruck Medical University VDVjekoslav DulićUniversity of North Carolina at Chapel Hill JSJoyce M. SlingerlandUniversity of Miami

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Implication

In vitro biochemical analysis demonstrates cell cycle-regulated inhibition of Cdk complexes by p28Ick in human cells, suggesting a broad family of G1/S transition regulators.

Key Points

To identify and characterize novel endogenous protein inhibitors that regulate human cyclin-dependent kinase (Cdk) complexes during the cell cycle.
Assayed human cell lysates to detect proteins that bind and inhibit preformed active Cdk/cyclin complexes.
Evaluated variations in inhibitory activity across different phases of the cell cycle and in G0- and G1-arrested cells.
Identified p28Ick, a 28-kDa protein that directly binds to and suppresses the catalytic kinase activity of preformed human Cdk/cyclin complexes.
Demonstrated that p28Ick inhibitory activity fluctuates during cell cycle progression, reaching peak levels in G1 and accumulating during G0 and G1 arrest.

Cite This Study

Hengst et al. (1994) studied this question.

synapsesocial.com/papers/6a82762bbde2ec1d675226fa https://doi.org/10.1073/pnas.91.12.5291

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Implication

In vitro biochemical analysis demonstrates cell cycle-regulated inhibition of Cdk complexes by p28Ick in human cells, suggesting a broad family of G1/S transition regulators.

A cell cycle-regulated inhibitor of cyclin-dependent kinases.

Discussion

Member takes

Implication

Key Points

Cite This Study

A cell cycle-regulated inhibitor of cyclin-dependent kinases.

Discussion

Member takes

Implication

Key Points

Cite This Study

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