What question did this study set out to answer?

To evaluate whether prostaglandin H2 functions as an endothelium-derived contracting factor and examine its regulatory interaction with endothelium-derived nitric oxide in hypertensive rat aorta.

August 1, 1991Journal of Hypertension

Prostaglandin H2 as an endothelium-derived contracting factor and its interaction with endothelium-derived nitric oxide

View Full Paper

Ask AI

Bookmark

Authors

TITakayuki ItoMerck (Germany)TKToshio KatôDaiyukai General Hospital YIYoshio IwamaNagoya University

Discussion

Member takes

Implication

Ex vivo study reveals that prostaglandin H2 triggers acetylcholine-induced vascular contraction in hypertensive rat aortas, indicating it functions as an endothelium-derived contracting factor.

Key Points

To evaluate whether prostaglandin H2 functions as an endothelium-derived contracting factor and examine its regulatory interaction with endothelium-derived nitric oxide in hypertensive rat aorta.
Mounted thoracic aortic rings isolated from spontaneously hypertensive rats in tissue baths, precontracted them with norepinephrine (10^-7 mol/l), and stimulated with acetylcholine (10^-5 mol/l).
Assessed pharmacological inhibition using the thromboxane A2/prostaglandin H2 receptor antagonist ONO-3708 and the nitric oxide synthase inhibitor N-nitroarginine methylester (NNM).
Compared vascular responses to exogenous prostaglandin H2 (5 x 10^-7 mol/l) and quantified 6-keto-prostaglandin F1 alpha production kinetics.
Acetylcholine induced a biphasic response featuring an initial relaxation peak at ~1 min followed by a contraction peak at ~7 min.
Pretreatment with ONO-3708 abolished the late contractile response to unmask sustained relaxation, whereas NNM completely prevented the relaxant phase.
In basal aortic rings pretreated with NNM, acetylcholine evoked a 7-min peak contraction that was abolished by ONO-3708, matching the contraction pattern produced by exogenous prostaglandin H2.

Cite This Study

Ito et al. (1991) studied this question.

synapsesocial.com/papers/6a828c085bcab2ccdb8d5b4f https://doi.org/10.1097/00004872-199108000-00006

View Full Paper

Ask AI

Bookmark

Implication

Ex vivo study reveals that prostaglandin H2 triggers acetylcholine-induced vascular contraction in hypertensive rat aortas, indicating it functions as an endothelium-derived contracting factor.

Prostaglandin H2 as an endothelium-derived contracting factor and its interaction with endothelium-derived nitric oxide

Discussion

Member takes

Implication

Key Points

Cite This Study

Prostaglandin H2 as an endothelium-derived contracting factor and its interaction with endothelium-derived nitric oxide

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider