Key result
A high-fat diet in New Zealand Obese mice induced obesity and hyperglycemia, which correlated with increased formation of immature vessels, though the direct increase in CD31-positive structures was not statistically significant.
Why the study?
Does a high-fat diet increase pathological angiogenesis in the New Zealand Obese mouse model?
Does a high-fat diet increase pathological angiogenesis in the New Zealand Obese mouse model?
p-value: p=not significant
A high-fat diet in New Zealand Obese mice exacerbates metabolic syndrome and promotes hyperglycemia-related activation of angiogenesis, leading to the formation of a pathological, immature vascular network.
High-fat diet links to immature vessels in obese mice; leaves open whether this drives pathological angiogenesis in human metabolic disease.
BACKGROUND: Obesity and its complications lead to vascular injury, atherosclerosis, diabetes and pathological angiogenesis. One of the models to study the obesity and its entanglements is the New Zealand Obese mice model. Aim of this study was to check the effect of high fat diet on changes in biochemical parameters as well as on process of angiogenesis in NZO mice. METHODS: NZO mice were fed with standard (ST) or high fat (HF) diet for seven weeks. Body weight and serum biochemical parameters were monitored. The PECAM1 positive vessel-like structures immunostaining, as well as the gene expression of the matrigel penetrating cells by microarray (confirmed by real-time PCR method) were analyzed. RESULTS: Mice fed with HF diet developed obesity. Number of newly created vessels with lumen was correlated with hyperglycemia and animal weight gain. The number of PECAM1 positive cells in matrigel tended to increase during HF diet. Microarray results revealed changes in gene expression (activation of the oxidative stress and insulin resistance, inhibition of apoptosis and cell differentiation), however without markers of endothelial cell network maturation. CONCLUSION: Observed changes in the NZO mice on HF diet argue for the hyperglycemia related activation of angiogenesis, leading to the formation of pathological, immature network.
No takes yet. Share an insight, caveat, or question.
Balwierz et al. (2009) studied Obesity and pathological angiogenesis (n=21). High fat diet vs. Standard rodent diet (3% fat) was evaluated on Number of CD31 (PECAM1) positive structures in matrigel plug (p=not significant). A high-fat diet in New Zealand Obese mice induced obesity and hyperglycemia, which correlated with increased formation of immature vessels, though the direct increase in CD31-positive structures was not statistically significant.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: