Key result
Antisense oligonucleotide inhibition of NCX reduced NCX expression by 90 +/- 4% and decreased the rise in intracellular Ca2+ during anoxia/reoxygenation by 95 +/- 3% in guinea pig ventricular myocytes.
Population
Cultured adult guinea pig ventricular myocytes
Design
Preclinical
Authors
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NCX inhibition attenuates Ca2+ overload in anoxic myocytes; hypothesis-generating for cardioprotection, with no clinical translation yet.
Inhibition of NCX expression by antisense oligonucleotides significantly reduces calcium overload during anoxia/reoxygenation in ventricular myocytes, suggesting NCX is the predominant mechanism for this overload.
Eigel et al. (2001) studied Anoxia/reoxygenation. Antisense oligonucleotide directed at guinea pig NCX1 was evaluated on Rise in intracellular Ca2+ concentration during anoxia/reoxygenation. Antisense oligonucleotide inhibition of NCX reduced NCX expression by 90 +/- 4% and decreased the rise in intracellular Ca2+ during anoxia/reoxygenation by 95 +/- 3% in guinea pig ventricular myocytes.
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