What is the clinical evidence from this study?

Study design: Other. Population: Insulin resistance. Intervention: High-sucrose, high-fat (HSHF) diet vs. Chow diet. Primary outcome: Adipose lipoprotein lipase (LPL) activity postprandially.

What question did this study set out to answer?

To determine whether adipose tissue lipoprotein lipase becomes resistant to insulin in a nutritional model of insulin resistance and to assess its impact on postprandial triglyceride clearance.

February 1, 2002AJP Endocrinology and Metabolism

Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet

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Key result

A high-sucrose, high-fat diet induced insulin resistance in adipose tissue lipoprotein lipase, leading to attenuated triglyceride clearance and heightened postprandial hypertriglyceridemia in rats.

Why the study?

Does a high-sucrose, high-fat diet induce insulin resistance in adipose tissue lipoprotein lipase in Sprague-Dawley rats?

Population

Sprague-Dawley rats

Comparison

Purified high-sucrose, high-fat diet for 4… vs Chow diet for 4 weeks, followed by 24 h fast and…

Design

Preclinical

Follow-up

4 weeks

Authors

FPFrédéric PicardLung Institute ABA. BoivinHéma-Québec JLJosée LalondeUniversity of Ottawa

Discussion

Member takes

Implication

May inform mechanisms of postprandial hypertriglyceridemia; leaves open translation to human dietary interventions.

Key Points

To determine whether adipose tissue lipoprotein lipase becomes resistant to insulin in a nutritional model of insulin resistance and to assess its impact on postprandial triglyceride clearance.
Sprague-Dawley rats were fed either standard chow or a purified high-sucrose, high-fat (HSHF) diet for 4 weeks to induce overt insulin resistance.
Following a 24-hour fast, rats were refed chow for 1, 3, or 6 hours to measure postprandial insulin, glucose, triglycerides, and tissue-specific lipoprotein lipase (LPL) activity.
Postprandial adipose LPL activity rose two- to fourfold in chow-fed rats but increased by only 30% in HSHF-fed rats, despite equivalent postprandial insulin levels and higher glycemia in the HSHF group.
Peak postprandial triglyceridemia was 70% higher in HSHF-fed rats than controls, while the rate of triglyceride entry into circulation was identical, indicating defective clearance.
Muscle LPL activity decreased postprandially in HSHF-fed animals while increasing in chow-fed controls.

Structured PICO

Does a high-sucrose, high-fat diet induce insulin resistance in adipose tissue lipoprotein lipase in Sprague-Dawley rats?

Population

Sprague-Dawley rats fed a purified high-sucrose, high-fat diet for 4 weeks to induce insulin resistance.

Intervention

Purified high-sucrose, high-fat (HSHF) diet for 4 weeks, followed by 24 h fast and refeeding for 1, 3, or 6 h

Comparator

Chow diet for 4 weeks, followed by 24 h fast and refeeding for 1, 3, or 6 h

Outcome

Adipose lipoprotein lipase (LPL) activity in response to refeeding/insulinsurrogate

Diet-induced insulin resistance impairs adipose tissue lipoprotein lipase activation, contributing to postprandial hypertriglyceridemia through attenuated triglyceride clearance.

Cite This Study

Picard et al. (2002) studied Insulin resistance. High-sucrose, high-fat (HSHF) diet vs. Chow diet was evaluated on Adipose lipoprotein lipase (LPL) activity postprandially. A high-sucrose, high-fat diet induced insulin resistance in adipose tissue lipoprotein lipase, leading to attenuated triglyceride clearance and heightened postprandial hypertriglyceridemia in rats.

synapsesocial.com/papers/6a830adc95b373e00c93c6e8 https://doi.org/10.1152/ajpendo.00307.2001

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Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider