We describe a system for accurate photometry and analysis of two‐dimensional (2‐D) gels, and its application to both high and low resolution gels. Image acquisition is video‐based and includes methods of data correction necessary for accurate photometry. The analysis is based upon a method described previously by Lutin et al. [8], which we have modified for improved stability and adapted to run on our PDP 11/60 computer. We illustrate analysis of a typical gel with a sequence of images showing the data while Gaussian components are estimated, removed, added and fitted as the iterative algorithm proceeds. We conclude by demonstrating the use of a mathematical curve‐of‐growth model for calibrating peak volumes vs. protein loading.
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Jansson et al. (1983) studied this question.
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