This study demonstrates a viable in vitro system of cultured mouse juxtaglomerular cells capable of producing renin and responding to beta-adrenergic stimulation with isoproterenol.
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Validates functional cultured JG cell model; leaves open in vivo translation and clinical relevance.
Trypsin- or collagenase-dispersed renal cortical cells from newborn mice were filtered and cultured. The cultures comprised 25% juxtaglomerular cells as identified by immunocytochemistry. Renin was measured by radioimmunoassay in the culture medium and in the cells at various time intervals. This in vitro system was responsive to isoproterenol, which stimulated renin release in a dose-dependent manner.
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Lacasse et al. (1987) studied this question.
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