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October 1, 1993Circulation ResearchOpen Access

Renin mRNA quantification using polymerase chain reaction in cultured juxtaglomerular cells. Short-term effects of cAMP on renin mRNA and secretion.

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Population

Cultured mouse renal juxtaglomerular cells

Design

Preclinical

Follow-up

up to 20 hours

Authors

RBRoberto Della BrunaEnte Ospedaliero CantonaleAKA. KurtzUniversity of BernPCPierre CorvolGeneral / Preventive / Lipids

Discussion

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Implication

cAMP potently activates renin transcription and secretion in vitro; leaves open relevance to human physiology or therapy.

Structured PICO

P
Population
Cultured mouse renal juxtaglomerular cells
I
Intervention
cAMP activators (forskolin, isoproterenol, isobutylmethylxanthine)
O
Outcome
Renin mRNA levels and renin secretionsurrogate

Quantitative PCR is a suitable tool for studying renin gene expression, demonstrating that cAMP is a potent and fast activator of renin gene transcription and secretion in juxtaglomerular cells.

Cite This Study

Bruna et al. (1993) studied this question.

synapsesocial.com/papers/6a86ac893f44447e627cab60https://doi.org/10.1161/01.res.73.4.639
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nonproportional changes in plasma renin concentration, renal renin content, and rat renin messenger RNA.1985 · 82 citations
  2. 2Control of renin secretion by Ca2+ and cyclic AMP through two parallel mechanisms1986 · 13 citations
  3. 3Measurement of Renin Activity, Concentration and Substrate in Rat Plasma by Radioimmunoassay of Angiotensin I1972 · 455 citations
  4. 4Illegitimate transcription: transcription of any gene in any cell type.1989 · 638 citations
  5. 5Regulation of Islet Somatostatin Secretion and Gene Expression: Selective Effects of Adenosine 3′,5′- Monophosphate and Phorbol Esters in Normal Islets of Langerhans and in a Somatostatin-Producing Rat Islet Clonal Cell Line 1027 B2*1991 · 27 citations