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November 1, 1993Journal of Biological ChemistryOpen Access

Cyclic AMP selectively increases renin mRNA stability in cultured juxtaglomerular granular cells.

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Why the study?

Does cAMP increase renin mRNA stability in cultured juxtaglomerular granular cells?

Population

Primary cultures of juxtaglomerular granular (JGG) cells isolated from mouse kidney

Comparison

Treatment with cAMP-elevating agents vs Incubation in the absence of cAMP-elevating agents

Design

Preclinical

Follow-up

Up to 24 hours

Authors

MCMing ChenJSJ. SchnermannASA Smart

Discussion

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Overview

Supports cAMP effects on renin expression in vitro; hypothesis-generating and should not alter clinical practice or guidelines.

Structured PICO

Does cAMP increase renin mRNA stability in cultured juxtaglomerular granular cells?

P
Population
Primary cultures of juxtaglomerular granular (JGG) cells isolated from mouse kidney
I
Intervention
Treatment with cAMP-elevating agents (forskolin, isoproterenol, 8-bromo-cAMP plus 3-isobutyl-1-methylxanthine, or cholera toxin)
C
Comparator
Incubation in the absence of cAMP-elevating agents
O
Outcome
Renin mRNA levels and mRNA half-lifesurrogate

cAMP increases renin release and mRNA levels in juxtaglomerular granular cells partly through a selective increase in renin mRNA stability.

Cite This Study

Chen et al. (1993) studied this question.

synapsesocial.com/papers/6a86aca67fdf9298f89eab85https://doi.org/10.1016/s0021-9258(20)80502-x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Juxtaglomerular cells grown as monolayer cell culture contain renin, angiotensin I-converting enzyme, and angiotensin I and II/III.1982 · 69 citations
  2. 2Molecular mechanism of tissue-specific regulation of mouse renin gene expression by cAMP. Identification of an inhibitory protein that binds nuclear transcriptional factor1991 · 58 citations
  3. 3Juxtaglomerular cells cultured on a reconstituted basement membrane1992 · 10 citations
  4. 4Time course of stimulation of renal renin messenger RNA by furosemide.1993 · 24 citations
  5. 5cAMP regulates transcription of the alpha 2A adrenergic receptor gene in HT-29 cells.1991 · 47 citations