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January 1, 2009General Physiology and BiophysicsOpen Access

Cyclic AMP increases cytoplasmic free calcium in renin-secreting cells from rat kidney

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Key result

In rat juxtaglomerular cells, increasing cAMP with agents like isoproterenol elicited an increase in intracellular free calcium that was approximately 40% of the response induced by angiotensin II.

Population

Sprague-Dawley rats on NaCl-depleted or control diet, from which glomeruli and afferent arterioles…

Comparison

cAMP-increasing agents including isoproterenol… vs Baseline conditions or angiotensin II stimulation

Design

Preclinical

Authors

JLJ. Laske-ErnsMCM. ChmielnickiUQUlrich Quast

Discussion

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Overview

Animal data link cAMP to partial Ca2+ mobilization in rat JG cells; leaves open its role in human renin regulation.

Structured PICO

P
Population
Isolated afferent arterioles from Sprague-Dawley rats kept on a NaCl-depleted diet to study renin-secreting juxtaglomerular cells.
I
Intervention
cAMP-increasing agents including isoproterenol, dibutyryl-cAMP, forskolin, and IBMX
C
Comparator
Baseline conditions or angiotensin II stimulation
O
Outcome
Intracellular free Ca2+ concentration ([Ca2+]i) measured by fura-2 fluorescence ratiosurrogate

Main Result

Effect estimate: 37 ± 8% of Angiotensin II response

In rat juxtaglomerular cells, cAMP activates store-operated Ca2+ channels to increase intracellular calcium, which may serve to finely adjust the stimulating effect of cAMP on the renin-angiotensin system.

Limitations

  • Absence of intravascular pressure in the isolated preparation results in a negative membrane potential and low resting calcium values.
  • The afferent arteriole is composed of smooth muscle cells and juxtaglomerular cells, making it difficult to be absolutely certain from which cell type the signal arises.
  • Absence of intravascular pressure in the isolated afferent arteriole preparation, resulting in negative membrane potential and low baseline [Ca2+]i
  • Preparation contains both smooth muscle cells and juxtaglomerular cells, making it difficult to be absolutely certain from which cell type the signal arises

Cite This Study

Laske-Erns et al. (2009) studied this question. cAMP-increasing agents (e.g., isoproterenol, dibutyryl-cAMP) vs. Angiotensin II (3 nmol/l) or baseline was evaluated on Peak increase in fura-2 fluorescence ratio R (intracellular free Ca2+) (37 ± 8% of Angiotensin II response). In rat juxtaglomerular cells, increasing cAMP with agents like isoproterenol elicited an increase in intracellular free calcium that was approximately 40% of the response induced by angiotensin II.

synapsesocial.com/papers/6a85aacdfc70cb81dc203907https://doi.org/10.4149/gpb_2009_04_404
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Decreased Intracellular Calcium Stimulates Renin Release via Calcium-Inhibitable Adenylyl Cyclase2006 · 79 citations
  2. 2Importance of calcium in renal renin release1986 · 17 citations
  3. 3Electrophysiology of the renin-producing juxtaglomerular cells2005 · 5 citations
  4. 4Adenylyl Cyclase Isoform V Mediates Renin Release From Juxtaglomerular Cells2006 · 62 citations
  5. 5Calcium-dependent phosphodiesterase 1C inhibits renin release from isolated juxtaglomerular cells2009 · 40 citations