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December 27, 2013AJP Renal Physiology

Angiotensin II stimulates basolateral 50-pS K channels in the thick ascending limb

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Population

Sprague-Dawley rats, specifically isolated medullary thick ascending limb tubules.

Comparison

Angiotensin II at concentrations ranging from 25… vs Control conditions and ANG II co-administered…

Design

Preclinical

Authors

MWMingxiao WangChongqing University of Posts and TelecommunicationsHLHaiyan LuanJiamusi UniversityPWPeng WuZhengzhou University

Discussion

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Overview

Hypothesis-generating for AT1R effects on renal K channels in animals; human relevance and therapeutic implications remain to be tested.

Structured PICO

P
Population
Sprague-Dawley (SD) rats (both sex, 60-80 g body weight), specifically isolated medullary thick ascending limb (TAL) tubules.
I
Intervention
Angiotensin II (ANG II) at concentrations ranging from 25 to 125 nM (primarily 50 nM) applied to isolated TAL tubules.
C
Comparator
Control conditions (no ANG II) and ANG II co-administered with various inhibitors (losartan, PD123319, U73122, calphostin C, tiron, DPI, herbimycin A, PP1).
O
Outcome
Basolateral 50-pS K channel activity (measured as NPo) and c-Src phosphorylation at tyrosine residue 416.surrogate

Angiotensin II stimulates basolateral 50-pS K channels in the thick ascending limb via AT1R through a PLC-PKC-NOX-dependent mechanism that activates Src-family kinases.

Cite This Study

Wang et al. (2013) studied this question.

synapsesocial.com/papers/6a8358aa629f3c7ed19bb60dhttps://doi.org/10.1152/ajprenal.00476.2013
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Also Consider

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