What question did this study set out to answer?

To investigate whether the ubiquitin-protein ligase Nedd4 mediates intracellular sodium-dependent feedback inhibition of epithelial sodium channels.

June 9, 1998Proceedings of the National Academy of Sciences

Nedd4 mediates control of an epithelial Na + channel in salivary duct cells by cytosolic Na +

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Authors

ADAnuwat DinudomThe University of Sydney KHKieran F. HarveyThe University of Melbourne PKP. KomwatanaThe University of Sydney

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Implication

Experimental study demonstrates that Nedd4 downregulates epithelial sodium channels in response to elevated intracellular sodium, indicating how regulatory defects cause Liddle syndrome.

Key Points

To investigate whether the ubiquitin-protein ligase Nedd4 mediates intracellular sodium-dependent feedback inhibition of epithelial sodium channels.
Assessed epithelial sodium channel regulation and feedback responses in salivary duct cells.
Investigated the functional dependence of channel downregulation on Nedd4, ubiquitin-mediated pathways, Go protein signaling, and cytosolic anion levels.
Nedd4 mediated ubiquitin-dependent downregulation of epithelial sodium channel activity triggered specifically by elevated intracellular sodium.
Nedd4 operated downstream of Go signaling in the sodium feedback cascade but showed no involvement in anion-dependent channel regulation.
Channel activity was unaffected by Nedd4 when sodium and anion feedback systems were inactive, attributing channel hyperactivity in Liddle's syndrome to the loss of this feedback mechanism.

Cite This Study

Dinudom et al. (1998) studied this question.

synapsesocial.com/papers/6a8358aa629f3c7ed19bb61d https://doi.org/10.1073/pnas.95.12.7169

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Implication

Experimental study demonstrates that Nedd4 downregulates epithelial sodium channels in response to elevated intracellular sodium, indicating how regulatory defects cause Liddle syndrome.

Nedd4 mediates control of an epithelial Na + channel in salivary duct cells by cytosolic Na +

Discussion

Member takes

Implication

Key Points

Cite This Study

Nedd4 mediates control of an epithelial Na + channel in salivary duct cells by cytosolic Na +

Discussion

Member takes

Implication

Key Points

Cite This Study

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