What question did this study set out to answer?

To determine whether steric hindrance between flanking splice sites prevents the inclusion of the short 18-nucleotide c-src N1 exon in non-neuronal cells.

March 1, 1991Genes & DevelopmentOpen Access

Does steric interference between splice sites block the splicing of a short c-src neuron-specific exon in non-neuronal cells?

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Authors

DBDouglas L. BlackUniversity of California, Los Angeles

Discussion

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Implication

In vitro study demonstrates length-dependent inclusion of the c-src N1 exon across cell types, suggesting steric interference between splice sites normally restricts non-neuronal splicing.

Key Points

To determine whether steric hindrance between flanking splice sites prevents the inclusion of the short 18-nucleotide c-src N1 exon in non-neuronal cells.
Assayed transiently expressed mouse c-src model genes for N1 exon insertion into mRNA in HeLa cells and LA-N-5 neuroblastoma cells.
Tested hybrid constructs inserting the N1 exon and flanking introns between adenovirus exons.
Engineered constructs extending the N1 exon length from 18 to 109 nucleotides and introduced mutations altering splice site strengths.
Normal c-src constructs omitted the N1 exon in HeLa cells but included it in 50% of mature mRNA in LA-N-5 cells, a neural specificity preserved even when placed between adenovirus exons.
Extending the N1 exon length to 109 nucleotides enabled efficient exon inclusion in HeLa cells.
Mutations altering the relative strength of flanking splice sites modified exon exclusion, consistent with a steric interference model.

Cite This Study

Douglas L. Black (1991) studied this question.

synapsesocial.com/papers/6a835965277f263e1a93409a https://doi.org/10.1101/gad.5.3.389

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March 1, 1991Genes & DevelopmentOpen Access

Does steric interference between splice sites block the splicing of a short c-src neuron-specific exon in non-neuronal cells?

View Full Paper

Ask AI

Bookmark

Authors

DBDouglas L. BlackUniversity of California, Los Angeles

Discussion

Member takes

Implication

In vitro study demonstrates length-dependent inclusion of the c-src N1 exon across cell types, suggesting steric interference between splice sites normally restricts non-neuronal splicing.

Key Points

To determine whether steric hindrance between flanking splice sites prevents the inclusion of the short 18-nucleotide c-src N1 exon in non-neuronal cells.
Assayed transiently expressed mouse c-src model genes for N1 exon insertion into mRNA in HeLa cells and LA-N-5 neuroblastoma cells.
Tested hybrid constructs inserting the N1 exon and flanking introns between adenovirus exons.
Engineered constructs extending the N1 exon length from 18 to 109 nucleotides and introduced mutations altering splice site strengths.
Normal c-src constructs omitted the N1 exon in HeLa cells but included it in 50% of mature mRNA in LA-N-5 cells, a neural specificity preserved even when placed between adenovirus exons.
Extending the N1 exon length to 109 nucleotides enabled efficient exon inclusion in HeLa cells.
Mutations altering the relative strength of flanking splice sites modified exon exclusion, consistent with a steric interference model.

Cite This Study

Douglas L. Black (1991) studied this question.

synapsesocial.com/papers/6a835965277f263e1a93409a https://doi.org/10.1101/gad.5.3.389

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