What question did this study set out to answer?

To evaluate whether chronically elevated plasma levels of atrial natriuretic peptide (ANP) cause down-regulation of ANP binding sites on platelets in patients with congestive cardiomyopathy.

October 1, 1988European Journal of Clinical Investigation

Binding sites for atrial natriuretic peptide on platelets in patients with congestive cardiomyopathy

View Full Paper

Ask AI

Bookmark

Why the study?

Are ANP binding sites on platelets down-regulated in patients with congestive cardiomyopathy and chronically elevated ANP plasma levels compared to healthy volunteers?

Population

15 adult patients with congestive cardiomyopathy and 18 healthy volunteers

Design

Case-control

Authors

TSTim M. StromDélégation Paris 5 JWJ. WeilUniversity of Memphis FBFabian BraunUniversität Hamburg

Discussion

Member takes

Implication

Questions expected ANP receptor down-regulation in cardiomyopathy; hypothesis-generating and should not change practice.

Key Points

To evaluate whether chronically elevated plasma levels of atrial natriuretic peptide (ANP) cause down-regulation of ANP binding sites on platelets in patients with congestive cardiomyopathy.
Assessed 15 adult patients with congestive cardiomyopathy in comparison to 18 healthy volunteers.
Quantified platelet ANP receptor density, receptor affinity (dissociation constants), and plasma levels of ANP and cyclic guanosine monophosphate (cGMP) using an assay capable of detecting total binding sites despite high circulating ANP.
Patients demonstrated markedly higher plasma ANP (median: 375 pg ml-1, range: 155–900 vs. median: 55.5 pg ml-1, range: 20–90) and plasma cGMP (median: 6.2 pmol ml-1, range: 2.5–21.4 vs. median: 1.8 pmol ml-1, range: 1–2.8) than healthy controls.
Platelet receptor density did not show a statistically significant reduction in patients (median: 19 receptors/platelet, range: 7.2–60.2) compared to healthy volunteers (median: 24.5 receptors/platelet, range: 14.8–41.1).
Binding affinity remained comparable between groups, with no significant difference in dissociation constants between patients (median: 10.5 pmol l-1, range: 7.9–27.4) and controls (median: 8.9 pmol l-1, range: 5.4–17).

Structured PICO

Are ANP binding sites on platelets down-regulated in patients with congestive cardiomyopathy and chronically elevated ANP plasma levels compared to healthy volunteers?

Population

15 adult patients with congestive cardiomyopathy and 18 healthy volunteers

Comparator

18 healthy volunteers

Outcome

Number of atrial natriuretic peptide (ANP) binding sites per plateletsurrogate

Patients with congestive cardiomyopathy do not exhibit down-regulation of ANP binding sites on platelets despite chronically elevated ANP plasma levels.

Cite This Study

Strom et al. (1988) studied this question.

synapsesocial.com/papers/6a83ba30ab0020f36262cdc7 https://doi.org/10.1111/j.1365-2362.1988.tb01051.x

View Full Paper

Ask AI

Bookmark

Binding sites for atrial natriuretic peptide on platelets in patients with congestive cardiomyopathy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Binding sites for atrial natriuretic peptide on platelets in patients with congestive cardiomyopathy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider