November 1, 1998AJP Heart and Circulatory Physiology
Assessment of glycogen turnover in aerobic, ischemic, and reperfused working rat hearts
Population
Isolated working rat hearts
Comparison
Low-flow ischemia followed by reperfusion vs Aerobic perfusion
Design
Preclinical
Follow-up
90 minutes (60 min ischemia + 30 min reperfusion)
Authors
Discussion
Loading...
Member takes
Implication
Supports further investigation of cardiac glycogen dynamics; leaves open translation to human ischemia-reperfusion injury.
Structured PICO
P
Population
Isolated working rat hearts
I
Intervention
Low-flow ischemia (0.5 ml/min, 60 min) followed by reperfusion (30 min)
C
Comparator
Aerobic perfusion (11 mM glucose, 1.2 mM palmitate, 100 microU/ml insulin)
O
Outcome
Glycogen turnover (rates of glycogen synthesis and degradation)surrogate
This novel dual-isotope method demonstrates that substantial glycogen turnover occurs simultaneously in the heart not only during aerobic conditions but also during ischemia and reperfusion.
Cite This Study
Fraser et al. (1998) studied this question.