What question did this study set out to answer?

To determine whether guanosine 3',5'-cyclic monophosphate (cGMP) acts as an intracellular inhibitory mediator of renin release in renal tissue.

September 1, 1988AJP Renal Physiology

Guanosine 3',5'-cyclic monophosphate as a mediator of inhibition of renin release

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Population

Rat superficial cortical slices and isolated cultured rat juxtaglomerular cells

Comparison

Atriopeptin III, nitroprusside, 8-bromoguanosine… vs Isoproterenol or arachidonic acid stimulation…

Design

Preclinical

Authors

WHWolfgang HenrichHumboldt-Universität zu Berlin EMElizabeth A. McAllisterUnited States Department of Veterans Affairs PSPhilip B. SmithWellesley College

Discussion

Member takes

Implication

Does not change practice; leaves open cGMP-mediated renin modulation as a research target pending human validation.

Key Points

To determine whether guanosine 3',5'-cyclic monophosphate (cGMP) acts as an intracellular inhibitory mediator of renin release in renal tissue.
Measured renin release and tissue cyclic nucleotide levels in rat superficial cortical slices exposed to isoproterenol, arachidonic acid, atriopeptin III, nitroprusside, 8-BrcGMP, and the guanylate cyclase inhibitor methylene blue.
Assessed renin secretion in isolated cultured rat juxtaglomerular cells treated with isoproterenol in the presence or absence of 8-BrcGMP.
In cortical slices, atriopeptin III, nitroprusside, and 8-BrcGMP abolished renin release stimulated by isoproterenol (10^-5 M) and arachidonic acid (10^-3 M); atriopeptin III and nitroprusside significantly increased tissue cGMP (P < 0.05) without altering cAMP.
Inhibition of guanylate cyclase by methylene blue (10^-5 M) abolished the inhibitory action of atriopeptin III on renin release, and in cultured juxtaglomerular cells, an isoproterenol-induced 58% increase in renin release (P < 0.01) was completely suppressed by 8-BrcGMP (10^-6 M).

Structured PICO

Population

Rat superficial cortical slices and isolated cultured rat juxtaglomerular cells

Intervention

Atriopeptin III (ANP), nitroprusside (NP), 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), and methylene blue

Comparator

Isoproterenol or arachidonic acid stimulation alone

Outcome

Renin releasesurrogate

This preclinical study demonstrates that cGMP acts as an intracellular mediator for the inhibition of renin release by ANP and nitroprusside.

Cite This Study

Henrich et al. (1988) studied this question.

synapsesocial.com/papers/6a83fa94b0b8dfdc4c62e772 https://doi.org/10.1152/ajprenal.1988.255.3.f474

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Guanosine 3',5'-cyclic monophosphate as a mediator of inhibition of renin release

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Guanosine 3',5'-cyclic monophosphate as a mediator of inhibition of renin release

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider