December 27, 2006Journal of VirologyOpen Access

Small Interfering RNA Targeted to Hepatitis C Virus 5′ NontranslatedRegion Exerts Potent Antiviral Effect

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Why the study?

Does shRNA targeting the 5' nontranslated region of the HCV genome inhibit virus replication in HCV-infected cells?

Structured PICO

Does shRNA targeting the 5' nontranslated region of the HCV genome inhibit virus replication in HCV-infected cells?

Population

Huh-7 and Huh-7.5 cells, including those persistently infected with HCV genotype 2a (JFH1), cell-culture-grown HCV genotype 1a (H77), and subgenomic/full-length replicons of HCV genotype 1b

Intervention

Vectors carrying short hairpin RNA (shRNA) targeting the 5' nontranslated conserved region of the HCV genome (specifically psh-53, psh-274, and psh-375)

Outcome

Inhibition of virus replication and HCV internal ribosome entry site (IRES)-mediated translationsurrogate

A conserved shRNA sequence (psh-274) targeting the HCV genome can inhibit replication of multiple HCV genotypes in vitro, suggesting potential as a therapeutic modality.

Discussion

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Implication

Supports preclinical shRNA development for HCV; leaves open translation to animal models or clinical use.

Cite This Study

Kanda et al. (2006) studied this question.

synapsesocial.com/papers/6a8418376b6022c7d8c52128 https://doi.org/10.1128/jvi.01496-06

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Small Interfering RNA Targeted to Hepatitis C Virus 5′ NontranslatedRegion Exerts Potent Antiviral Effect

Structured PICO

Discussion

Implication

Cite This Study

Small Interfering RNA Targeted to Hepatitis C Virus 5′ NontranslatedRegion Exerts Potent Antiviral Effect

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider