Key result
Direct delivery of HCV IRES shRNAs efficiently blocked HCV IRES-mediated firefly luciferase expression in transfected human 293FT cells and a mouse model.
Why the study?
Do shRNAs targeting the HCV IRES inhibit HCV IRES-mediated gene expression in human cells and a mouse model?
Population
Human 293FT cells and a mouse model (hydrodynamic transfection via tail vein)
Design
Preclinical
Authors
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Animal data support shRNA for HCV; leaves open human efficacy and safety.
Do shRNAs targeting the HCV IRES inhibit HCV IRES-mediated gene expression in human cells and a mouse model?
shRNAs targeting the HCV IRES can efficiently inhibit HCV IRES-mediated gene expression in vitro and in vivo, suggesting potential as a novel therapeutic approach for HCV infections.
Wang et al. (2005) studied Hepatitis C virus (HCV) infection. Small hairpin interfering RNAs (shRNAs) targeting the HCV IRES element was evaluated on HCV IRES-mediated firefly luciferase (fLuc) expression. Direct delivery of HCV IRES shRNAs efficiently blocked HCV IRES-mediated firefly luciferase expression in transfected human 293FT cells and a mouse model.
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