Why the study?
Does chronic stimulation reduce myosin light chain kinase activity in rabbit fast muscle?
Does chronic stimulation reduce myosin light chain kinase activity in rabbit fast muscle?
Chronic stimulation of fast twitch muscle induces a time-dependent decrease in MLCK activity that precedes myosin isoform conversion, consistent with fast-to-slow muscle transformation.
Hypothesis-generating for activity-dependent myosin remodeling in rabbits; leaves open translation to human cardiac or skeletal muscle plasticity.
Analysis of myosin light chain kinase (MLCK) activity in tibialis anterior muscles of the rabbit revealed that chronic stimulation at a frequency of 10 Hz for 24 h per day reduced the enzyme activity in a time-dependent manner. Since fast twitch muscle contains significantly more myosin light chain kinase than slow twitch muscle, the observed reductions are consistent with the type of fast-to-slow transformation observed for other type-specific muscle characteristics. The present data also indicate that the stimulation-induced decrease in MLCK activity precedes the fast-to-slow conversion of the myosin molecule as judged by pyrophosphate-polyacrylamide gel electrophoresis.
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Klug et al. (1986) studied this question.
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