November 1, 1988Proceedings of the National Academy of SciencesOpen Access

Addition of a foreign oligopeptide to the major capsid protein of poliovirus.

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Population

Type 3 poliovirus cDNA transfected into Vero and HEp-2 cells, and immunized rabbits.

Comparison

Insertion of a tri- or hexapeptide into the… vs Parental Sabin strain (P3/Leon 12a1b).

Design

Preclinical

Authors

FCFlorence Colbère-GarapinInserm CCChristina ChristodoulouFrederick University RCR CrainicCentre National de la Recherche Scientifique

Discussion

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Implication

Poliovirus epitope engineering is feasible in rabbits; leaves open translation to human vaccines or multivalent platforms.

Structured PICO

Population

Type 3 poliovirus (Sabin strain) cDNA (P3/Leon 12a1b) transfected into Vero and HEp-2 cells, and immunized rabbits.

Intervention

Insertion of a tri- or hexapeptide (foreign oligopeptide) into the major capsid protein VP1 at residue 100 (neutralization site 1).

Comparator

Parental Sabin strain (P3/Leon 12a1b).

Outcome

Virus viability, phenotypic markers (plaque size, heat stability, growth cycle), and antigenic/immunogenic properties.

Insertion of a foreign oligopeptide into the major capsid protein of poliovirus creates a viable mutant with a new, highly immunogenic antigenic site.

Cite This Study

Colbère-Garapin et al. (1988) studied this question.

synapsesocial.com/papers/6a845eb0c0e58a00207bf8ab https://doi.org/10.1073/pnas.85.22.8668

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Addition of a foreign oligopeptide to the major capsid protein of poliovirus.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Addition of a foreign oligopeptide to the major capsid protein of poliovirus.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider