April 1, 1997Journal of Clinical InvestigationOpen Access

In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat.

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Why the study?

Does in vivo gene transfection with human HSP70 improve myocardial tolerance to ischemia-reperfusion injury in rats?

Population

Sprague-Dawley rats, n=52.

Comparison

Intracoronary infusion of hemagglutinating virus… vs Control hearts infused with HVJ-liposome without…

Design

Preclinical

Follow-up

4 days after gene transfection; 24 hours after heat stress

Authors

KSKeiichiro SuzukiSalk Institute for Biological Studies YSYoshiki SawaCardiac Surgery YKYasufumi KanedaThe University of Osaka

Discussion

Member takes

Implication

HSP70 gene transfection bolsters myocardial ischemia-reperfusion tolerance in rats; leaves open translation to clinical cardioprotection.

Structured PICO

Does in vivo gene transfection with human HSP70 improve myocardial tolerance to ischemia-reperfusion injury in rats?

Population

Sprague-Dawley rats (250 g), n=52 (13 per group: HSP70 gene transfected, control transfected, heat-stressed, and nontreated).

Intervention

Intracoronary infusion of hemagglutinating virus of Japan (HVJ)-liposome containing human HSP70 gene, followed by heterotopic transplantation for 4 days.

Comparator

Control hearts infused with HVJ-liposome without the HSP70 gene; whole-body heat-stressed rats (42°C for 20 min); and nontreated rats.

Outcome

Myocardial tolerance to ischemia-reperfusion injury (functional recovery of left ventricular developed pressure [LVDP], max dP/dt, min dP/dt, coronary flow, and creatine phosphokinase [CPK] leakage) after 30 min of global ischemia and 60 min of reperfusion in a Langendorff apparatus.surrogate

In vivo gene transfection with HSP70 significantly enhances myocardial tolerance to ischemia-reperfusion injury in rats, suggesting a potential novel method for myocardial protection.

Cite This Study

Suzuki et al. (1997) studied this question.

synapsesocial.com/papers/6a848135a47fb2e1cdb2ae93 https://doi.org/10.1172/jci119327

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In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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