Involvement of cGMP-dependent protein kinase in the relaxation of ovine pulmonary arteries to cGMP and cAMP

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Why the study?

Does protein kinase G (PKG) mediate cGMP- and cAMP-induced relaxation in isolated ovine pulmonary arteries?

Structured PICO

Does protein kinase G (PKG) mediate cGMP- and cAMP-induced relaxation in isolated ovine pulmonary arteries?

Population

Isolated pulmonary arteries of lambs (age = 10 +/- 1 days), preconstricted with endothelin-1

Intervention

8-bromo-cGMP (8-BrcGMP) or 8-BrcAMP in the presence of selective inhibitors of PKG (Rp-8-PET-BrcGMPS) or PKA (KT-5720)

Comparator

Absence of PKG or PKA inhibitors

Outcome

Relaxation responses of isolated pulmonary arteriessurrogate

This preclinical study demonstrates that protein kinase G plays a crucial role in both cGMP- and cAMP-mediated relaxation of pulmonary arteries.

No takes yet. Share an insight, caveat, or question.

May support PKG as a target in pulmonary vasodilation; hypothesis-generating and leaves clinical translation open.

Dhanakoti et al. (2000) studied this question.

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