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January 1, 2001Blood

Actin reorganization and proplatelet formation in murine megakaryocytes: the role of protein kinase Cα

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Why the study?

Does inhibition of PKCalpha prevent proplatelet formation in murine megakaryocytes?

Population

Mature megakaryocytes isolated from suspension murine bone marrow cell cultures

Design

Preclinical

Authors

PRPonlapat RojnuckarinChulalongkorn UniversityKKKenneth KaushanskyStony Brook School

Discussion

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Implication

PKCα inhibition selectively blocks proplatelet formation in mice; leaves open whether this pathway can be targeted to modulate human thrombopoiesis.

Structured PICO

Does inhibition of PKCalpha prevent proplatelet formation in murine megakaryocytes?

P
Population
Mature megakaryocytes isolated from suspension murine bone marrow cell cultures
I
Intervention
Inhibition of protein kinase C (PKC) activation (via bis-indolylmaleimide I, phorbol myristate acetate, or PKCalpha-specific ribozymes), MAP kinases, PI3 kinase, protein kinase A, integrin signaling (disintegrin), or actin polymerization (cytochalasin D)
O
Outcome
Proplatelet formation and actin reorganizationsurrogate

PKCalpha plays a critical role in proplatelet development by altering actin dynamics in megakaryocytes, providing a potential target for pharmacologic manipulation of thrombopoiesis.

Cite This Study

Rojnuckarin et al. (2001) studied this question.

synapsesocial.com/papers/6a85215d76469a949f43d161https://doi.org/10.1182/blood.v97.1.154
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The role of megakaryocyte growth and development factor in terminal stages of thrombopoiesis1996 · 102 citations
  2. 2Thrombopoietin-Induced Activation of the Mitogen-Activated Protein Kinase (MAPK) Pathway in Normal Megakaryocytes: Role in Endomitosis1999 · 214 citations
  3. 3The Response of Megakaryocytes with Processes to Thrombin1987 · 26 citations
  4. 4Actin-Dependent Propulsion of Endosomes and Lysosomes by Recruitment of N-Wasp✪2000 · 458 citations
  5. 5Effects of Cytokines on Platelet Production From Blood and Marrow CD34+ Cells1998 · 127 citations