Does nitric oxide allow endothelial cells to sense hypoxia and mediate hypoxic vasodilatation?in vivo and in vitro studies
Population
Anaesthetised rats (in vivo) and superfused endothelial cells (in vitro)
Comparison
Systemic hypoxia, NO synthesis blockade with… vs Normoxia, baseline conditions
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Should not change clinical hypoxia management; hypothesis-generating for NO-adenosine endothelial O2 sensing in vascular research.
Structured PICO
P
Population
Anaesthetised rats (in vivo) and superfused endothelial cells (in vitro)
I
Intervention
Systemic hypoxia, NO synthesis blockade with L-NAME, NO donor infusion, adenosine A(1) receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine, adenosine infusion, 8-bromo-cGMP, bradykinin
C
Comparator
Normoxia, baseline conditions
O
Outcome
Femoral vascular conductance (FVC, hindlimb vasodilatation) and adenosine releasesurrogate
In vivo, shear-released NO may increase the apparent Km of endothelial cytochrome oxidase for O2, allowing the endothelium to act as an O2 sensor and release adenosine in response to moderate hypoxia.
Cite This Study
Edmunds et al. (2003) studied this question.