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June 1, 2021Open Access

Development of a robust and simple in vitro assay (RSR) that allows modification of ribosomes without compromising other key translational components.

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Why the study?

How stress-modified ribosomes regulate translation remains elusive due to the lack of an appropriate experimental system without off-target effects of modifying agents.

Population

Yeast ribosomes and translational machinery constituents

Design

In vitro assay development and validation study

Authors

BTBrandon M. TrainorDPDimitri G. PestovNSNatalia Shcherbik

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Overview

Enables isolated study of stress-modified ribosomes in vitro; leaves open translation to cardiovascular models.

Structured PICO

P
Population
Yeast ribosomes and in vitro translation system
I
Intervention
Ribosome separation and reconstitution assay (RSR)
C
Comparator
Canonical approaches based on exposing cells or cell-free extracts to stressors
O
Outcome
Separation of yeast ribosomes from other translational machinery constituents, followed by reconstitution of the translation reaction

The RSR assay provides a novel in vitro method to study the impact of stress-modified ribosomes on translation without off-target effects.

Cite This Study

Trainor et al. (2021) studied this question.

synapsesocial.com/papers/6a855f7473102ab9cdb5549fhttps://doi.org/10.1101/2021.05.31.446494
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