November 11, 2003Molecular and Cellular BiologyOpen Access

Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine Tracts

View Full Paper

Ask AI

Bookmark

Population

In vitro splicing models (plasmids, HeLa nuclear extract, recombinant proteins) studying HIV-1 splicing

Comparison

HIV-1 hnRNP A/B-dependent ESS, ESSV vs Mutant ESSVx or conditions without hnRNP A/B or…

Design

Preclinical

Authors

JDJeffrey K. DomsicUniversity of Iowa YWYibin WangRutgers, The State University of New Jersey AMAkila MayedaFujita Health University

Discussion

Member takes

Implication

Hypothesis-generating for HIV splicing-targeted antivirals; leaves open translation from this animal model mechanism.

Structured PICO

Population

In vitro splicing models (plasmids, HeLa nuclear extract, recombinant proteins) studying HIV-1 splicing

Intervention

HIV-1 hnRNP A/B-dependent ESS, ESSV

Comparator

Mutant ESSVx or conditions without hnRNP A/B or U2AF

Outcome

Spliceosome assembly (E complex formation) and binding of U2AF65 to polypyrimidine tracts

This study elucidates the molecular mechanism by which HIV-1 ESSV represses splicing, showing it blocks early spliceosome assembly by preventing U2AF65 binding.

Cite This Study

Domsic et al. (2003) studied this question.

synapsesocial.com/papers/6a85816d37bcb8373dada71f https://doi.org/10.1128/mcb.23.23.8762-8772.2003

View Full Paper

Ask AI

Bookmark

Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine Tracts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine Tracts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider