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January 1, 2010Biological and Pharmaceutical BulletinOpen Access

Calcium Channel Inhibitor, Verapamil, Inhibits the Voltage-Dependent K+ Channels in Rabbit Coronary Smooth Muscle Cells

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Population

Single vascular smooth muscle cells isolated from the left anterior descending coronary artery of New…

Comparison

Verapamil (0.3 to 30 microM) applied in vitro. vs Control conditions and nifedipine.

Design

Preclinical

Authors

EKEun‐A KoJeju National UniversityWPWon Sun ParkKangwon National UniversityYSYoun Kyoung SonGachon University

Discussion

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Implication

Verapamil may inhibit coronary Kv channels beyond Ca2+ blockade; extends mechanistic insight in myocytes but should not change practice.

Structured PICO

P
Population
Single vascular smooth muscle cells isolated from the left anterior descending coronary artery of New Zealand White rabbits (2.0-2.3 kg, either sex).
I
Intervention
Verapamil (0.3 to 30 microM) applied in vitro.
C
Comparator
Control conditions (absence of verapamil) and nifedipine (10 microM).
O
Outcome
Inhibition of voltage-dependent K+ (Kv) channel currents measured via whole-cell patch clamp.surrogate

Verapamil directly inhibits voltage-dependent K+ channels in coronary arterial smooth muscle cells in a state-, time-, and use-dependent manner, independent of its known Ca2+ channel blocking activity.

Limitations

  • Detailed molecular subtypes of Kv channel remain to be determined
  • Needs further experiments using overexpressed HEK-293 cells or siRNA knockdown to confirm specific Kv subtypes affected

Cite This Study

Ko et al. (2010) studied this question.

synapsesocial.com/papers/6a85910f37bcb8373dadabbfhttps://doi.org/10.1248/bpb.33.47
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Mechanism of Block and Identification of the Verapamil Binding Domain to HERG Potassium Channels1999 · 374 citations
  2. 2Comparative Cardiovascular Actions of Clentiazem, Diltiazem, Verapamil, Nifedipine, and Nimodipine in Isolated Rabbit Tissues1992 · 19 citations
  3. 3Heteromultimeric Kv1.2-Kv1.5 Channels Underlie 4-Aminopyridine-Sensitive Delayed Rectifier K<sup>+</sup>Current of Rabbit Vascular Myocytes2001 · 86 citations
  4. 4Inhibition of voltage-gated K<sup>+</sup>current in rat intrapulmonary arterial myocytes by endothelin-11998 · 118 citations
  5. 5Ca(2+)‐activated and voltage‐gated K+ currents in smooth muscle cells isolated from human mesenteric arteries.1992 · 80 citations