December 15, 1994The Journal of Cell BiologyOpen Access

Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.

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Population

Dictyostelium discoideum RMLC null cells (mLCR-)

Comparison

Expression of mutant RMLC from an actin promoter… vs Expression of wild-type RMLC

Design

Preclinical

Authors

BOBruce OstrowGrand Valley State University LCLan S. ChenNorthwestern University RCRex L. ChisholmNorthwestern University

Discussion

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Implication

Challenges necessity of RMLC phosphorylation for myosin function; leaves open relevance to human cardiac myocytes.

Structured PICO

Population

Dictyostelium discoideum RMLC null cells (mLCR-)

Intervention

Expression of mutant RMLC (ser13ala substitution) from an actin promoter on an integrating vector

Comparator

Expression of wild-type RMLC

Outcome

Rescue of primary phenotypic defects (cytokinesis and developmental defects)surrogate

Phosphorylation of the Dictyostelium regulatory light chain of myosin is not essential for myosin-dependent cellular functions such as cytokinesis and development.

Cite This Study

Ostrow et al. (1994) studied this question.

synapsesocial.com/papers/6a85bccefc70cb81dc203f60 https://doi.org/10.1083/jcb.127.6.1945

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Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider