Phosphorylation of the myosin regulatory light chain plays a role in motility and polarity duringDictyosteliumchemotaxis

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Population

Dictyostelium discoideum cells (wild-type and S13A mutants expressing nonphosphorylatable RLC in the RLC…

Comparison

Mutation of the single phosphorylatable serine… vs Wild-type cells

Design

Preclinical

Authors

Discussion

No immediate clinical implications; leaves open whether myosin regulatory light chain phosphorylation regulates chemotaxis in mammalian cells.

Population

Dictyostelium discoideum cells (wild-type and S13A mutants expressing nonphosphorylatable RLC in the RLC null mutant, mlcR(-))

Intervention

Mutation of the single phosphorylatable serine to a nonphosphorylatable alanine (S13A) in the myosin regulatory light chain (RLC)

Comparator

Wild-type cells

Outcome

Motile behavior and chemotactic responsiveness to spatial, temporal, and concentration components of natural cAMP wavessurrogate

Phosphorylation of the myosin regulatory light chain is required for cellular depolarization and efficient chemotaxis in Dictyostelium discoideum.

Zhang et al. (2002) studied this question.

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No immediate clinical implications; leaves open whether myosin regulatory light chain phosphorylation regulates chemotaxis in mammalian cells.