July 1, 2013PLoS ONEOpen Access

Improvement in Renal Hemodynamics following Combined Angiotensin II Infusion and AT1R Blockade in Aged Female Sheep following Fetal Unilateral Nephrectomy

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Why the study?

Does AT1R blockade and AngII infusion improve renal hemodynamics in aged female sheep with fetal unilateral nephrectomy?

Population

10 pure-bred Australian merino female sheep (5 fetal uninephrectomised, 5 sham control) at 5 years of age.

Comparison

Angiotensin II infusion, followed by AT1R… vs Sham control group receiving the same infusions.

Design

Preclinical

Follow-up

5 years of age

Authors

RSReetu R. SinghCentral University of Punjab YLYugeesh R. LankadevaUniversity of Kelaniya KDKate M. DentonDiscovery Institute

Discussion

Member takes

Implication

AT2R stimulation may improve renal hemodynamics in nephron-deficit models; leaves open translation to human renoprotection.

Structured PICO

Does AT1R blockade and AngII infusion improve renal hemodynamics in aged female sheep with fetal unilateral nephrectomy?

Population

10 pure-bred Australian merino female sheep (5 fetal uninephrectomised, 5 sham control) at 5 years of age.

Intervention

Angiotensin II (AngII) infusion (0.2 mg/kg/h i.v. for 90 min), followed by AT1R blockade via losartan (10 ml bolus 0.95 mg/kg, then 1.9 mg/kg/h i.v. infusion), and AngII in the presence of losartan.

Comparator

Sham control group (5 singleton female fetuses where left kidney was cleared from fat but not excised) receiving the same infusions.

Outcome

Renal hemodynamic responses (renal blood flow, renal vascular resistance, glomerular filtration rate) and tubular sodium excretion.surrogate

Increasing the availability of AngII to AT2R by AT1R blockade improved renal blood flow in a sheep model of congenital nephron deficit, suggesting AT2R as a potential therapeutic target.

Limitations

Gene and protein expression do not always correlate, and protein levels were not measured due to lack of specific commercial antibodies.

Cite This Study

Singh et al. (2013) studied this question.

synapsesocial.com/papers/6a8651eada4bf993812ed422 https://doi.org/10.1371/journal.pone.0068036

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Improvement in Renal Hemodynamics following Combined Angiotensin II Infusion and AT1R Blockade in Aged Female Sheep following Fetal Unilateral Nephrectomy

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Improvement in Renal Hemodynamics following Combined Angiotensin II Infusion and AT1R Blockade in Aged Female Sheep following Fetal Unilateral Nephrectomy

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider