What question did this study set out to answer?

To sequence and analyze the viral replicase gene and the 3' noncoding region of the coxsackievirus B3 genome.

September 1, 1984Journal of VirologyOpen Access

Replicase gene of coxsackievirus B3

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Population

Coxsackievirus B3 genome (cDNA clone covering two-thirds of the genome in pBR322 vector)

Comparison

Nucleotide sequencing of the gene for viral… vs Poliovirus 1 and swine vesicular disease virus…

Design

Preclinical

Authors

PSPer StålhandskeUppsala University MLMichael LindbergGeorgetown University UPU. PetterssonUmeå University

Discussion

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Implication

Homology data extends enterovirus genomics; leaves open whether replicase conservation informs human pathogenesis or antiviral strategies.

Key Points

To sequence and analyze the viral replicase gene and the 3' noncoding region of the coxsackievirus B3 genome.
Cloned a cDNA fragment representing two-thirds of the coxsackievirus B3 genome into the PstI site of plasmid vector pBR322.
Determined the nucleotide sequence of the viral replicase gene and the adjacent 3' noncoding region.
Demonstrated that the predicted amino acid sequence of the coxsackievirus B3 replicase is remarkably similar to that of poliovirus 1 replicase.
Found weak homology between the coxsackievirus B3 3' noncoding region and poliovirus 1, but close homology to swine vesicular disease virus.
Identified a 13-nucleotide sequence near the polyadenylic acid junction that is conserved across multiple enteroviruses.

Structured PICO

Population

Coxsackievirus B3 genome (cDNA clone covering two-thirds of the genome in pBR322 vector)

Intervention

Nucleotide sequencing of the gene for viral replicase and 3' noncoding region

Comparator

Poliovirus 1 and swine vesicular disease virus sequences

Outcome

Nucleotide and predicted amino acid sequence homology

The study identifies the nucleotide sequence of the coxsackievirus B3 replicase gene, revealing significant homology with poliovirus 1 and identifying a conserved 13-nucleotide segment near the polyadenylic acid junction.

Cite This Study

Stålhandske et al. (1984) studied this question.

synapsesocial.com/papers/6a865533e7c8470ab14ca560 https://doi.org/10.1128/jvi.51.3.742-746.1984

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Implication

Homology data extends enterovirus genomics; leaves open whether replicase conservation informs human pathogenesis or antiviral strategies.

Replicase gene of coxsackievirus B3

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Replicase gene of coxsackievirus B3

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider