November 1, 1999Journal of VirologyOpen Access

Requirements for RNA Replication of a Poliovirus Replicon by Coxsackievirus B3 RNA Polymerase

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Population

HeLa cells transfected with chimeric poliovirus type 1 (PV1) genome

Design

Preclinical

Authors

YBYolanda C. BellUniversity of California, Irvine BSBert L. SemlerUniversity of California, Irvine EEEllie EhrenfeldNational Institutes of Health

Discussion

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Implication

Chimeric replication defects highlight strict 3CD-P2/P3 compatibility needs; leaves open whether engineering can overcome genus barriers.

Structured PICO

Population

HeLa cells transfected with chimeric poliovirus type 1 (PV1) genome

Intervention

Replacement of 3D RNA polymerase (3D(pol)) coding sequences or 3CD sequences with those from coxsackievirus B3 (CVB3)

Outcome

Infectious virus production and RNA replicationsurrogate

There are essential interactions between 3CD and other viral P2 or P3 protein products required for efficient RNA replication that are not fully achieved between proteins from different members of the same virus genus.

Cite This Study

Bell et al. (1999) studied this question.

synapsesocial.com/papers/6a7c48267eebcb99d4a16132 https://doi.org/10.1128/jvi.73.11.9413-9421.1999

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Requirements for RNA Replication of a Poliovirus Replicon by Coxsackievirus B3 RNA Polymerase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Requirements for RNA Replication of a Poliovirus Replicon by Coxsackievirus B3 RNA Polymerase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider