April 15, 2001Journal of VirologyOpen Access

Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis

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Population

HeLa cells transfected with plasmids encoding poliovirus nonstructural proteins

Comparison

Transfection with plasmids producing viral RNAs… vs Plasmids producing viral RNAs designed to serve…

Design

Preclinical

Authors

NTNatalya L. TeterinaLomonosov Moscow State University DEDenise EggerUniversity of Bern KBKurt BienzUniversity of Basel

Discussion

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Implication

Identifies potential antiviral targets in viral RNA replication; extends mechanistic insights but leaves open human translation.

Structured PICO

Population

HeLa cells transfected with plasmids encoding poliovirus nonstructural proteins

Intervention

Transfection with plasmids producing viral RNAs with mutations (substitution of PV 5' NCR with EMCV IRES, or insertion of 3 nucleotides in 3Dpol coding sequence)

Comparator

Plasmids producing viral RNAs designed to serve as templates for replication (wild-type)

Outcome

Assembly of poliovirus replication complexes, intracellular membrane reorganization, viral RNA localization, and negative-strand RNA synthesissurrogate

The 5'-terminal sequences of poliovirus RNA are essential for initiating minus-strand RNA synthesis at the 3' end.

Cite This Study

Teterina et al. (2001) studied this question.

synapsesocial.com/papers/6a8175169f1055894da8a68d https://doi.org/10.1128/jvi.75.8.3841-3850.2001

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Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider