Tryptic digestion of peptide A, the C‐terminal fragment of thioredoxin, obtained by cyanogen bromide cleavage, resulted in the isolation of nine peptides. The complete amino acid sequence of each was determined, and the N‐terminal and C‐terminal fragments of peptide A were identified. Together the nine tryptic peptides accounted for all 71 residues of peptide A. Hydrazinolysis and studies with carboxypeptidase A established ‐Asn‐Leu‐Ala to be the C‐terminal sequence of peptide A and of thioredoxin.
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Arne Holmgren (1968) studied this question.