What question did this study set out to answer?

To determine the roles of adenosine A1 and A2 receptor subtypes and ATP-dependent potassium channels in protecting against myocardial stunning during repeated coronary artery occlusions.

July 1, 1993CirculationOpen Access

Glibenclamide antagonizes adenosine A1 receptor-mediated cardioprotection in stunned canine myocardium.

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Population

Barbital-anesthetized dogs subjected to six 5-minute periods of coronary occlusion interspersed with…

Comparison

Pretreatment with selective adenosine A1… vs Control conditions

Design

Preclinical

Follow-up

2 hours of reperfusion

Authors

ZYZhenhai YaoHua Hong Semiconductor (China)GGG J GrossMedical College of Wisconsin

Discussion

Member takes

Implication

A1 agonism may confer cardioprotection in animal ischemia models; leaves open clinical translation and KATP mediation in humans.

Key Points

To determine the roles of adenosine A1 and A2 receptor subtypes and ATP-dependent potassium channels in protecting against myocardial stunning during repeated coronary artery occlusions.
Assessed barbital-anesthetized dogs subjected to six 5-minute coronary artery occlusions interspersed with 10-minute reperfusion intervals, followed by 2 hours of final reperfusion.
Measured regional myocardial segment shortening using sonomicrometry following administration of selective A1 agonist CPA, A1 antagonist DPCPX, A2 agonist CGS 21680, and KATP blocker glibenclamide.
Pretreatment with the A1 agonist CPA improved percent segment shortening throughout reperfusion, whereas the A1 antagonist DPCPX significantly worsened postischemic functional recovery.
Administration of the A2 agonist CGS 21680 had no measurable effect on the recovery of percent segment shortening.
Low-dose glibenclamide (0.1 mg/kg) alone did not alter segment shortening, but it completely abolished the functional recovery provided by CPA, independent of hemodynamics, collateral flow, or ischemic bed size.

Structured PICO

Population

Barbital-anesthetized dogs subjected to six 5-minute periods of coronary occlusion interspersed with 10-minute periods of reperfusion and ultimately followed by 2 hours of reperfusion

Intervention

Pretreatment with selective adenosine A1 receptor antagonist DPCPX (1.0 mg/kg i.v. bolus), agonist CPA (2.0 micrograms.kg-1.min-1 i.v. infusion), selective A2 receptor agonist CGS 21680 (0.2 micrograms.kg-1.min-1), or KATP channel blocker glibenclamide (0.1 mg/kg)

Comparator

Control conditions

Outcome

Regional segment function (percent segment shortening) measured with sonomicrometry throughout reperfusionsurrogate

Stimulation of myocardial adenosine A1 receptors is cardioprotective during repetitive brief coronary occlusions, partially mediated via opening of myocardial KATP channels.

Cite This Study

Yao et al. (1993) studied this question.

synapsesocial.com/papers/6a86a078073460dfde2ab10c https://doi.org/10.1161/01.cir.88.1.235

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Glibenclamide antagonizes adenosine A1 receptor-mediated cardioprotection in stunned canine myocardium.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Glibenclamide antagonizes adenosine A1 receptor-mediated cardioprotection in stunned canine myocardium.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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