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September 18, 2010BloodOpen Access

A G184S substitution in Gi2α enhanced platelet aggregation in vitro and increased platelet accumulation after vascular injury, demonstrating an active role for RGS proteins in regulating platelets.

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Population

Mice with a G184S substitution in Gi2α

Comparison

G184S substitution in Gi2α blocking RGS/Gi2… vs Wild-type/control mice (implied)

Design

Preclinical

Key result

A G184S substitution in Gi2α enhanced platelet aggregation in vitro and increased platelet accumulation after vascular injury, demonstrating an active role for RGS proteins in regulating platelets.

Authors

RSRachel S. SignarvicACAleksandra CierniewskaTSTimothy J. Stalker

Discussion

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Overview

Should not yet inform clinical platelet management; extends preclinical evidence for RGS proteins in hemostasis.

Structured PICO

P
Population
Mice with a G184S substitution in Gi2α (expressed either as a global knock-in or limited to hematopoietic cells)
E
Exposure
G184S substitution in Gi2α blocking RGS/Gi2 interactions
C
Comparator
Wild-type/control mice (implied)
O
Outcome
Platelet aggregation in vitro and platelet accumulation after vascular injurysurrogate

RGS proteins actively regulate platelet responsiveness and limit the magnitude of the normal hemostatic response by modulating Gi2-dependent signaling.

Cite This Study

Signarvic et al. (2010) studied Platelet activation and thrombus formation. G184S substitution in Gi2α (blocking RGS/Gi2 interactions) vs. Wild-type (implied) was evaluated on Platelet aggregation in vitro and platelet accumulation after vascular injury. A G184S substitution in Gi2α enhanced platelet aggregation in vitro and increased platelet accumulation after vascular injury, demonstrating an active role for RGS proteins in regulating platelets.

synapsesocial.com/papers/6a86cae424dcec08efbef02ehttps://doi.org/10.1182/blood-2010-05-283846
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